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ABOUT US

Dr Dalia Marija KOPUSTINSKIENĖ

Research Scientist
Institute of Neurosciences
Lithuanian University of Health Sciences.

Lecturer
Department of Biochemistry
Lithuanian University of Health Sciences.

Invited Lecturer
Center of Postgraduate Studies for Farmacy Students
Lithuanian University of Health Sciences.

Doctoral research project at Habil Dr A. Toleikis laboratory, Institute for Biomedical Research, Kaunas University of Medicine, Kaunas, Lithuania (October, 1998 - June, 2002).

E-mail: daliammk[at]mail[dot]lt

RESEARCH INTERESTS

Protective and toxic effects of natural substances and drugs on cellular energy turover, ion fluxes in mitochondria and mechanisms of apoptosis.
Molecular modelling in drug discovery and design.

LABORATORY TECHNIQUE EXPERIENCE

Protein chemistry techniques: absorbtion spectroscopy methods (determination the amount of cytochromes c + c1, and cytochrome a in mitochondria and heart mucle fibers).
Cellular bioenergetics techniques: isolation of mitochondria from heart, skeletal muscle and liver, preparation of skinned heart and skeletal muscle fibers;fluorimetric, oxygraphic and ionometric techniques.
Molecular biology techniques: DNA and RNA extraction, PCR and RT-PCR.

COLLABORATION

Prof. Nils-Erik Saris, Department of Applied Chemistry and Microbiology, Viikki Biocenter 1, University of Helsinki, Finland. Research visits in: 2000 (4 months), 2001 (2 months), 2003 (2 months).

SELECTED PUBLICATIONS

Liobikas J, Majiene D, Trumbeckaite S, Kursvietiene L, Masteikova R, Kopustinskiene DM, Savickas A, Bernatoniene J.
Uncoupling and antioxidant effects of ursolic acid in isolated rat heart mitochondria. J. Nat. Prod., 2011, 74(7):1640-1644.
Bernatoniene J, Majiene D, Peciura R, Laukeviciene A, Bernatoniene R, Mekas T, Kasauskas A, Kopustinskiene D.
The effect of Ginkgo biloba extract on mitochondrial oxidative phosphorylation in the normal and ischemic rat heart. Phytother. Res., 2011, 25(7): 1054-1060.
Kopustinskiene DM, Liobikas J, Skemiene K, Malinauskas F, Toleikis A.
Direct effects of K(ATP) channel openers pinacidil and diazoxide on oxidative phosphorylation of mitochondria in situ. Cell. Physiol. Biochem., 2010, 25(2-3):181-186.
Ziemys A, Toleikis A, Kopustinskiene DM
Molecular modelling of K(ATP) channel blockers-ADP/ATP carrier interactions. IEE Proc. Syst. Biol., 2006, 153 (5): 390-393.
Kopustinskas A, Adaskevicius R, Krusinskas A, Kopustinskiene DM, Liobikas J, Toleikis A
A user-friendly PC-based data acquisition and analysis system for respirometric investigations. Comput. Methods Programs Biomed., 2006, 82 (3): 231-237.
Kopustinskiene DM, Pollesello P Saris N-E
Potassium-specific effects of levosimendan on heart mitochondria. Biochem. Pharmacol., 2004, 68 (5): 807-812.
Kopustinskiene DM, Toleikis A and Saris N-E
Adenine nucleotide translocase mediates the K(ATP) channel-openers-induced proton and potassium flux to the mitochondrial matrix. J. Bioenerg. Biomembr., 2003, 35 (2): 141-148.
Kopustinskiene DM, Jovaisiene J, Liobikas J and Toleikis A
Diazoxide and pinacidil uncouple pyruvate+malate induced mitochondrial respiration. J. Bioenerg. Biomembr., 2002, 34 (1): 49-53.
Kopustinskiene DM, Pollesello P, Saris N-EL
Levosimendan is a mitochondrial K(ATP) channel opener. Eur. J. Pharmacol., 2001, 428 (3): 311-314.
Liobikas J, Kopustinskiene DM, Toleikis A
What controls the outer mitochondrial membrane permeability for ADP: facts for and agains the role of oncotic pressure. Biochim. Biophys. Acta, 2001, 1505: 220-225.

CONTACT INFO

        Laboratory of Biochemistry, Institute of Neurosciences, Medical Academy, Lithuanian University of Health Sciences,
        Eiveniu str. 4, LT-50009 Kaunas, LITHUANIA.
        Fax: +370 37 302 959

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The page updated on 02 02 2012.