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METHODS
Feel free to use the protocols.

GEL ELECTROPHORESIS OF PROTEINS

• Resolving and stacking gel solutions for SDS-PAGE (18.5 cm x 20 cm gels).
  
  
• Resolving and stacking gel solutions for SDS-PAGE (7.5 cm x 10 cm gels).
  
  
• Laemmli sample and running buffers for SDS-PAGE.
  
  
• The extraction of mitochondrial proteins and the chemicals and solutions for isoelectric focusing .
  
  
• Separation of mitochondrial integral membrane proteins from soluble and membrane-associated proteins by salt extraction and carbonate wash.
  
  
• The composition of IEF sample solution.
  
  
• Preparation of samples for SDS-PAGE by precipitation with trichloroacetic acid (TCA) and acetone.
  
  
• Blue Native polyacrylamide gel electrophoresis: BN-PAGE.
  
  
• Silver, Coomassie and SYPRO Ruby staining and destaining of proteins in polyacrylamide gels.
  
  
• In-gel alkylation and digestion of proteins with endoproteinase Glu-C (V8) and modified trypsin.

 MASS SPECTROMETRY (MALDI-TOF MS)

• Preparation of samples for MALDI-TOF MS without desalting.

WESTERN BLOTTING

• The buffers for Western blotting.
  
  
• The home-made ECL Western blotting detection reagents.
  
  
• Western blotting.
  
  
• Staining the blot.

POLYMERISATION AND NEGATIVE STAINING OF PROTEINS

• Actin.
  
  
• Catalase.
  
  
• Glutamic acid dehydrogenase (L-GDH).

MITOCHONDRIA AND OXIDATIVE PHOSPHORYLATION

• The Biuret reagent and the determination of mitochondrial protein concentration.
  
  
• The preparation of rat (and rabbit) saponin and saponin plus collagenase treated heart muscle fibers.
  
  
• The preparation of rat saponin and saponin plus collagenase treated skeletal muscle fibers.
  
  
• The isolation of rat heart and liver mitochondria.

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